Rethinking High-Content Imaging: Myths vs. Realities

High-content imaging (HCI) is transforming life science research, offering valuable insights into cellular behavior, drug discovery, and disease mechanisms. Yet despite its growing impact, persistent myths can limit how researchers apply and benefit from this technology. In this free article, we separate fact from fiction, providing practical guidance for optimizing your imaging workflows.

Inside you’ll learn how to:

• Better understand how all components of a light path affect image quality, and not just the type of excitation source. 
• Achieve higher multiplexing with fewer, carefully selected laser lines instead of adding more.
• Optimize acquisition settings by adjusting magnification, field of view and z-stack settings to match your assay goals. 
• Leverage AI-powered image analysis while understanding its limitations, including transparency and data bias considerations.

Download the full eBook to rethink what’s possible with modern high-content imaging.