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Amerigo Scientific

SEAP Assay kit -for Transfection Assay | Amerigo Scientific

OZ Biosciences SEAP Assay Kit(Secreted Alkaline Phosphatase) is a colorimetric assay for sensitive quantification of SEAP in culture medium from transfected cells or tissues. Alkaline phosphatase cleaves the colourless 4-nitrophenylphosphate (pNPP) substrate to give a yellow p-nitrophenolate product in alkaline solutions. It iscommonly used as a reporter gene expression and can be accurately quantified. It is a truncated form of placental alkaline phosphatase (GPI-anchored protein).

The kit contains sufficient reagents to perform 500 assays in 96-well plate format./

SEAP assay kit Main Features:

Designed to detect SEAP reporter gene expression

Fast and easy analysis

Colorimetric – measure with a standard spectrophotometer or an ELISA reader (405 nm)

Multiple samples can be analyzed in low volumes

Convenient and economical packaging: no tablet, no powder/

Sizes :pNPP-containing Assay Buffer component A: 10 x 1 mL

Reaction Buffer #1 component B: 125 mL

Reaction Buffer #2 component C: 125 µL

Dilution Buffer component D: 50 mL

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ABOUT THE COMPANY

Amerigo Scientific is a distribution company focused on providing critical products and services to the biomedical and life science research communities. Although a new company, Amerigo Scientific's founder has had more than 20 years of rich experience in the biomedical and biochemical fields, and has established close contacts with key personnel in...
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