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Amerigo Scientific

Shrimp Alkaline Phosphatase | Amerigo Scientific

Developed at ArcticZymes, first launched in 1993. Originates from the Arctic Shrimp (Pandalus borealis), from 2010 it has been produced as a recombinant version (rSAP). rSAP is better suited than native SAP (nSAP) for newer, more sensitive technologies.

Shrimp Alkaline Phosphatase (rSAP) has become one of today’s most-selling DNA modifying enzymes due to the added convenience through a complete heat inactivation. While most other alkaline phosphatases (from E. coli or Calf intestine) must be removed by extraction procedures, rSAP is completely inactivated after 15 minutes at 65°C. rSAP works well in common buffers without the requirement for other additions.

Main advantages with rSAP

• Very high specific activity

• 100% heat-inactivated at 65°C

• Removes 5’-phosphates from DNA, RNA, dNTPs and proteins

• May be added directly to restriction enzyme digests

• No vector purification necessary

• Requires no supplemental zinc or other additives for activity

• Works directly in many different buffers

• Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis

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ABOUT THE COMPANY

Amerigo Scientific is a distribution company focused on providing critical products and services to the biomedical and life science research communities. Although a new company, Amerigo Scientific's founder has had more than 20 years of rich experience in the biomedical and biochemical fields, and has established close contacts with key personnel in...
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