Companies / Amerigo Scientific / HL-Exol - 3’-5’ Exonuclease
Amerigo Scientific

HL-Exol - 3’-5’ Exonuclease | Amerigo Scientific

HL-ExoI is a 3’-5’ exonuclease, specific for single stranded DNA. HL-ExoI is active at temperatures ≥25°C, and inactivated by 1 min incubation at 80°C, or 15 min at 60°C. Recombinantly produced in an E. coli strain expressing the HL-ExoI gene originating from an Arctic marine bacterium.

Unit definition: One unit catalyses the release of 10 nmol of acid soluble nucleotides in 30 min at 25°C. Reaction buffer: 20 mM Tris-HCl pH 7.5 at 25°C, 200 mM NaCl, 20 mM MgCl2, 1 mM DTT, 0.01% Triton X-100, 2.5% glycerol and 3H-dATP-labelled DNA (25 ng/µl).

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ABOUT THE COMPANY

Amerigo Scientific is a distribution company focused on providing critical products and services to the biomedical and life science research communities. Although a new company, Amerigo Scientific's founder has had more than 20 years of rich experience in the biomedical and biochemical fields, and has established close contacts with key personnel in...
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