The Power of Autofluorescence Extraction Using Full Spectrum Profiling™

On a conventional flow cytometer, researchers might be forced to use dyes emitting in spectral regions not affected by AF. But with Full Spectrum Profiling™ (FSP™) as found on the Cytek® Aurora, Northern Lights, and Aurora CS, investigators can fully understand AF, extract the AF signal, and ultimately improve their data resolution.

We will explore different examples of samples with high levels of AF that benefit from Cytek's FSP technology. By fully characterizing and subsequently extracting the AF signature across the entire spectrum, we will show how the resolution of dim markers is improved, sometimes even revealing populations previously masked by cellular AF.