1. This assay has been developed to screen GOAT activity by monitoring Acylated Ghrelin production in cell culture media. Cells over-expressing GOAT are cultivated in large excess of Unacylated Ghrelin, GOAT transforming Unacylated Ghrelin into Acylated Ghrelin. The kit detects Acylated Ghrelin with 103 to 105 more Unacylated Ghrelin in the sample.
2. Alternatively, it can be used for measuring Acylated Ghrelin in human biological samples. For this application, please contact technical support at CD.