The monoclonal antibody JHD-7.10.1 reacts with human complement factor H related proteins 1 and 2 (CFHR1/2). In humans, the complement factor H protein family consists of complement factor H, factor H-like protein 1, and five factor H-related proteins (called 1 to 5). All members of this group are mainly expressed in the liver and then secreted into the plasma. The secreted form of these proteins consists only of repetitive protein domains called short consensus repeats (SCR). The CFHR1 protein is composed of 5 SCR domains and circulates in plasma in two differentially glycosylated subtypes (37 kDa and 43 kDa). CFHR-2 consists of four SCRs, which are present in human plasma in non-glycosylated (24 kDa) and 29 kDa glycosylated forms. So far, little is known about the biological functions of CFHR-1 and CFHR-2 proteins, but recent studies have shown that CFHR1 is a complement regulator that can block the activity of C5 convertase and the assembly of terminal components and membrane insertion. In addition, genetic variations in the CFHR1 gene are associated with several diseases, such as atypical hemolytic uremic syndrome and age-related macular degeneration. Due to the high degree of amino acid sequence homology, monoclonal antibodies can cross-react with CFHR1 and 2. This antibody is suitable for immunoassay and Western blot applications.